DETERMINATION OF PRILOCAINE HCl IN BULK DRUG AND PHARMACEUTICAL FORMULATION BY GC-NPD METHOD

作者：Atila Alptug; Kadioglu Yucel^*

来源：Chemical Industry & Chemical Engineering Quarterly, 2013, 19(2): 313-319.

DOI：10.2298/CICEQ120124064A

• GC-NPD
• prilocaine HCl
• pharmaceutical formulation
• validation

A novel analytical method was developed and validated for determination of prilocaine HCl in bulk drug and pharmaceutical formulation by gas chromatography-nitrogen phosphorus detection (GC-NPD). The chromatographic separation was performed using a HP-5MS column. The calibration curve was linear over the concentration range of 40-1000 ng ml(-1) with a correlation coefficient of 0.9998. The limits of detection (LOD) and quantification (LOQ) of the method were 10 and 35 ng m(-1), respectively. The within-day and between-day precision, expressed as the percent relative standard deviation (RSD%) were less than 5.0%, and the accuracy (percent relative error) was better than 4.0%. The developed method can be directly and easily applied for determination of prilocaine HCl in bulk drug and pharmaceutical formulation using internal standard methodology.

• 出版日期2013-6