Acrobeloides buetschlii as a potential vector for enteric pathogens

作者：Kroupitski Yulia; Pinto Riky; Bucki Patricia; Belausov Edward; Ruess Liliane; Spiegel Yitzhak; Sela Shlomo^*

来源：Nematology, 2015, 17(4): 447-457.

DOI：10.1163/15685411-00002880

• Escherichia coli
• human pathogens
• interaction
• nematode behaviour
• Salmonella enterica

The objective of the present study was to assess if Acrobeloides buetschlii, an opportunistic species common across many soils, can be employed as a suitable model for interactions between free-living soil nematodes and enteric human pathogens. Acrobeloides buetschlii was exposed to mCherry-tagged Salmonella enterica and Escherichia coli O157:H7 and its vector potential was assessed. Salmonella cells were more readily ingested by the nematodes compared to E. coli O157:H7. Adult nematodes ingested more bacteria compared to juveniles. Salmonella survived internally for at least 7 days without affecting the viability of nematodes. Bacterial ingestion by A. buetschlii did not vary for three tested Salmonella serovars but was significantly lower for E. coli O157:H7. Considering the ubiquitous nature of pathogen and vector, these findings suggest that A. buetschlii can serve as a relevant model for studying nematode-Salmonella interactions in an agricultural setting and as potential transport for food-borne pathogens from soil to crops.

• 出版日期2015