Phospholipase C-zeta-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injectiond

作者：Swann Karl^*; Windsor Shane; Campbell Karen; Elgmati Khalil; Nomikos Michail; Zernicka Goetz Magdalena; Amso Nazar; Lai Anthony; Thomas Adrian; Graham Christopher

来源：Fertility and Sterility, 2012, 97(3): 742-747.

DOI：10.1016/j.fertnstert.2011.12.013

• Oocyte
• zygote
• calcium
• movement
• phospholipase C zeta
• cross correlation
• particle image velocimetry

Objective: To evaluate the imaging of cytoplasmic movements in human oocytes as a potential method to monitor the pattern of Ca2+ oscillations during activation. %26lt;br%26gt;Design: Test of a laboratory technique. %26lt;br%26gt;Setting: University medical school research laboratory. %26lt;br%26gt;Patient(s): Donated unfertilized human oocytes from intracytoplasmic sperm injection (ICSI) cycles. %26lt;br%26gt;Intervention(s): Microinjection of oocytes with phospholipase C (PLC) zeta (zeta) cRNA and a Ca2+-sensitive fluorescent dye. %26lt;br%26gt;Main Outcome Measure(s): Simultaneous detection of oocyte cytoplasmic movements using particle image velocimetry (PIV) and of Ca2+ oscillations using a Ca2+-sensitive fluorescent dye. %26lt;br%26gt;Result(s): Microinjection of PLC zeta cRNA into human oocytes that had failed to fertilize after ICSI resulted in the appearance of prolonged Ca2+ oscillations. Each transient Ca2+ concentration change was accompanied by a small coordinated movement of the cytoplasm that could be detected using PIV analysis. %26lt;br%26gt;Conclusion(s): The occurrence and frequency of cytoplasmic Ca2+ oscillations, a critical parameter in activating human zygotes, can be monitored by PIV analysis of cytoplasmic movements. This simple method provides a novel, noninvasive approach to determine in real time the occurrence and frequency of Ca2+ oscillations in human zygotes. (Fertil Steril (R) 2012; 97: 742-7.

• 出版日期2012-3