Negative Regulation of Hypoxic Responses via Induced Reptin Methylation

作者：Lee Jason S; Kim Yunho; Kim Ik Soo; Kim Bogyou; Choi Hee June; Lee Ji Min; Shin Hi Jai R; Kim Jung Hwa; Kim Ji Young; Seo Sang Beom; Lee Ho; Binda Olivier; Gozani Or; Semenza Gregg L; Kim Minhyung; Il Kim Keun; Hwang Daehee; Baek Sung Hee^*

来源：Molecular Cell, 2010, 39(1): 71-85.

DOI：10.1016/j.molcel.2010.06.008

Lysine methylation within histones is crucial for transcriptional regulation and thus links chromatin states to biological outcomes. Although recent studies have extended lysine methylation to nonhistone proteins, underlying molecular mechanisms such as the upstream signaling cascade that induces lysine methylation and downstream target genes modulated by this modification have not been elucidated. Here, we show that Reptin, a chromatin-remodeling factor, is methylated at lysine 67 in hypoxic conditions by the methyltransferase G9a. Methylated Reptin binds to the promoters of a subset of hypoxia-responsive genes and negatively regulates transcription of these genes to modulate cellular responses to hypoxia.

• 出版日期2010-7-9