Antibacterial activity of petroleum ether fraction from Laminaria japonica extracts against Clavibacter michiganensis subsp sepedonicus

作者：Cai Jin; Feng Jia; Wang Feipeng; Xu Qiufeng; Xie Shulian^*

来源：European Journal of Plant Pathology, 2014, 140(2): 291-300.

DOI：10.1007/s10658-014-0462-1

• Laminaria japonica
• Petroleum ether fraction
• Clavibacter michiganensis subsp sepedonicus
• Antibacterial activity
• Chemical composition
• Ultrastructural changes

We prepared an ethanol extract from Laminaria japonica, and obtained five fractions from the extract using various solvents. We investigated the antibacterial activities of the fractions against Clavibacter michiganensis subsp. sepedonicus (Spieckermann & Kotthoff) Davis et al., the causal agent of bacterial ring rot of potato (BRR). Among all the fractions, the petroleum ether fraction showed the strongest (P < 0.05) antibacterial activity against C. michiganensis subsp. sepedonicus. We analyzed the petroleum ether fraction to detect the components with antibacterial activity, and identified 24 compounds. The main constituents of the petroleum ether extract were esters (35.28 %) and acids (44.84 %). Cell membrane permeability assays, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analyses revealed that the petroleum ether fraction caused drastic ultrastructural changes in the cells of the pathogen. The petroleum ether fraction caused envelope disruption, cell deformation, the separation of the cell wall from the cell membrane, and the formation of vacuoles. These results clearly indicate that the petroleum ether fraction obtained from L. japonica shows strong antibacterial activity against the causal agent of BRR.

• 出版日期2014-10
• 单位山西大学