Antinociceptive Effects of (1 -> 3),(1 -> 6)-Linked beta-Glucan Isolated From Pleurotus pulmonarius in Models of Acute and Neuropathic Pain in Mice: Evidence for a Role for Glutamatergic Receptors and Cytokine Pathways

作者：Baggio Cristiane Hatsuko; Freitas Cristina Setim; Martins Daniel Fernandes; Mazzardo Leidiane; Smiderle Fhernanda Ribeiro; Sassaki Guilherme Lanzi; Iacomini Marcello; Andrade Marques Maria Consuelo; Soares Santos Adair Roberto

来源：Journal of Pain, 2010, 11(10): 965-971.

DOI：10.1016/j.jpain.2010.01.005

• beta-Glucan
• Pleurotus pulmonarius
• nociception
• glutamate
• cytokine

The present study evaluated the antinociceptive effect of (1 -> 3),(1 -> 6)-linked beta-glucan (GL) isolated from Pleurotus pulmonarius (Fr.) Quel. in mice and its possible mechanism of action. Intraperitoneal administration of GL inhibited glutamate-induced licking with an ID(50) of 0.34 mg/kg and inhibition of 96% +/- 3%. The treatment of animals with GL (1 mg/kg i.p.) inhibited nociception induced by intrathecal injection of N-methyl-D-aspartic acid, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, kainate and interleukin -1 beta in 67% +/- 13%, 89% +/- 11%, 74% +/- 9%, and 75% +/- 7%, respectively, but not the nociceptive response induced by (+/-)-1-aminocyclopentane-trans-1,3-dicarboxylic acid, substance P. and tumor necrosis factor-alpha. Moreover, GL (30 mg/kg i.p.) also reduced mechanical allodynia caused by partial sciatic nerve ligation for 2 hours, with inhibition of 47% +/- 10% observed 0.5 hours after treatment. When given chronically (twice a day) over 7 days, GL reversed the mechanical allodynia caused by partial sciatic nerve ligation (inhibition of 45% +/- 13% to 60% +/- 8%). Interestingly, GL did not affect the locomotor activity of mice in an open field test with doses that produce antinociceptive effects. Our findings show that GL inhibits acute and neuropathic pain in mice through mechanisms that involve the inhibition of ionotropic glutamate receptors and the interleukin -1 beta pathway.
Perspective: This article presents the antinociceptive activity of GL in acute and neuropathic pain with participation of ionotropic glutamate receptors and pro-inflammatory cytokines (interleukin-1 beta). After further experiments, this compound may represent a new pharmacological agent for the treatment of clinical pain.

• 出版日期2010-10