科研之友
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摘要
A novel strategy for dual-channel detection of metallothioneins (MTs) and Hg2+ has been proposed. In the absence of Hg2+, the functional chimera aptamer (FCA) designed can form an intact G-quadruplex with flexibility, which was demonstrated to have peroxidase-like activities upon hemin binding. In the presence of Hg2+, the formation of T-Hg2+-T complex results in the conformational switching of FCA, which lost the peroxidase-like activities and cannot catalyze the oxidation of ABTS by H2O2. Upon addition of MTs in this solution, MTs could interact with Hg2+ to form a MTs-Hg2+ complex, leading to the recovery of the G-quadruplex DNAzyme. The color and absorbance of the sensing system were also changed accordingly. In the optimizing condition, Delta A was directly proportional to the concentration ranging from 8.84 nM to 1.0 mu M for Hg2+, and 7.82 nM to 0.462 mu M for MTs with the detection limits of 2.65 nM and 2.34 nM, respectively. The proposed dual-channel method avoids the label steps in common methods, and allows direct analysis of the samples without costly instruments, and is reliable, inexpensive and sensitive.
- 出版日期2015-3-25
- 单位南华大学
