Anti-arthritic properties of crude extract from Chenopodium ambrosioides L. leaves

作者:Pereira Wanderson S; da Silva Girlaine P; Vigliano Mariana V; Leal Nathalia R F; Pinto Fabiana A; Fernandes Daniele C; Santos Shirley V M; Martino Thiago; Nascimento Johnny R; de Azevedo Ana Paula S; Fonseca Eduardo N; Velozo Leosvaldo S M; Souza Neto Lauro R; Bastos Frederico F; Portari Elyzabeth A; Carvalho Sabino Katia C; Nascimento Flavia; Coelho Marsen G P*
来源:Journal of Pharmacy and Pharmacology, 2018, 70(8): 1078-1091.
DOI:10.1111/jphp.12926

摘要

ObjectivesTo evaluate the effect of hydroalcoholic crude extract (HCE) from Chenopodium ambrosioides leaves on the development of type II collagen-induced arthritis (CIA) and on pro-inflammatory cytokine balance.
MethodsCollagen-induced arthritis was induced in DBA1/J mice. On the 21st day, the mice were treated orally with HCE or methotrexate, daily. Six weeks after beginning the treatment, the following measures were determined: lymphoid organs cell numbers, percentage of blood cells, IL-6, IFN-, TNF- and IL-17 serum concentrations, activity of hepatic and kidney glutathione S-transferase, hepatic 7-ethoxyresorufin-O-deethylase activity, bone density and histopathology.
Key findingsTreatment of CIA mice with HCE 5mg/kg (HCE5) reduced the percentage of neutrophils and macrophages and the number of bone marrow cells and increased the lymphocyte numbers and the inguinal lymph node cellularity. This treatment inhibited the serum concentration of IL-6 and TNF-, which may be related to the preservation of bone density and to the slight thickening of periarticular tissues, with minimal fibrosis and fibroblast proliferation in the joints. The CIA group presented advanced articular erosion and synovial hyperplasia. Phytochemical analysis showed mainly flavonols.
ConclusionsHCE5 presented anti-arthritic potential and reduced IL-6 and TNF-, which participate directly in the development and maintenance of the inflammatory process in rheumatoid arthritis.

  • 出版日期2018-8