Ang-(1-7) promotes the migration and invasion of human renal cell carcinoma cells via Mas-mediated AKT signaling pathway

作者：Zheng, Shuai; Yang, Ying; Song, Ran; Yang, Xiaomei; Liu, Hua; Ma, Qian; Yang, Longyan; Meng, Ran; Tao, Tao; Wang, Songlin; He, Junqi^*

来源：Biochemical and Biophysical Research Communications, 2015, 460(2): 333-340.

DOI：10.1016/j.bbrc.2015.03.035

• Renal cell carcinoma
• Angiotensin-(1-7)
• Mas receptor
• Migration
• Invasion
• AKT signaling

Ang-(1-7) is an active peptide component of renin-angiotensin system and endogenous ligand for Mas receptor. In the current study, we showed that Ang-(1-7) enhanced migratory and invasive abilities of renal cell carcinoma cells 786-O and Caki-1 by wound-healing, transwell migration and transwell invasion assays. Mas antagonist A779 pretreatment or shRNA-mediated Mas knockdown abolished the stimulatory effect of Ang-(1-7). Furthermore, Ang-(1-7)-stimulated AKT activation was inhibited by either A779 pretreatment or Mas knockdown. Blockage of AICT signaling by AICT inhibitor VIII inhibited Ang-(1-7)-induced migration and invasion in 786-O cells. Taken together, our results provided the first evidence for the pro-metastatic role of Ang-(1-7) in RCC, which may help to better understand the molecular mechanism underlying the progression of this tumor.

• 出版日期2015-5-1
• 单位首都医科大学