Determination of lead, cadmium and mercury in microwave-digested foodstuffs by RP-HPLC with an on-line enrichment technique

作者：Huang ZJ; Yang GY^*; Hu QF; Yin JY

来源：Analytical Sciences, 2003, 19(2): 255-258.

DOI：10.2116/analsci.19.255

A new method for the simultaneous determination of lead, cadmium and mercury ions in microwave-digested foodstuffs by reversed-phase high-performance liquid chromatography combined with on-line enrichment technique has been developed. The foodstuff samples were digested by microwave digestion. The lead, cadmium and mercury ions can be precolumn derivatized with 5,10,15,20-tetrakis(3-aminophenyl)porphine (T(3)APP) to form color chelates; then, the Hg-T(3)APP, Cd-T(3)APP and Pb-T(3)APP chelates can be enriched and separated on a valve switching HPLC system combined with on-line enrichment technique. The linearity ranges are 0.01 - 120 mug/l for each metal ion. The detection limits (S/N = 3) of lead, cadmium and mercury are 1.2 ng/l, 0.5 ng/l and 0.8 ng/l, respectively. This method was applied to the determination of lead, cadmium and mercury in foodstuffs with good results.

• 出版日期2003-2
• 单位云南大学