A fluorescence scanning electron microscope

作者：Kanemaru Takaaki^*; Hirata Kazuho; Takasu Shin ichi; Isobe Shin ichiro; Mizuki Keiji; Mataka Shuntaro; Nakamura Kei ichiro

来源：Materials Today, 2010, 12: 18-23.

DOI：10.1016/S1369-7021(10)70141-3

Fluorescence techniques are widely used in biological research to examine molecular localization, while electron microscopy can provide unique ultrastructural information. To date, correlative images from both fluorescence and electron microscopy have been obtained separately using two different instruments, i.e. a fluorescence microscope (FM) and an electron microscope (EM). In the current study, a scanning electron microscope (SEM) (JEOL JXA8600 M) was combined with a fluorescence digital camera microscope unit and this hybrid instrument was named a fluorescence SEM (FL-SEM). In the labeling of FL-SEM samples, both Fluolid, which is an organic EL dye, and Alexa Fluor, were employed. We successfully demonstrated that the FL-SEM is a simple and practical tool for correlative fluorescence and electron microscopy.

• 出版日期2010