Development of a continuous assay and steady-state characterization of Escherichia coli threonine synthase

作者：Morneau Dominique J K; Abouassaf Edgar; Skanes Jennifer E; Aitken Susan M^*

来源：Analytical Biochemistry, 2012, 423(1): 78-85.

DOI：10.1016/j.ab.2012.01.004

• Pyridoxal 5 %26apos
• -phosphate
• Threonine and methionine biosynthesis
• Threonine deaminase
• Hydroxyisocaproate dehydrogenase

Threonine synthase (TS) catalyzes the hydrolysis of O-phospho-L-homoserine (OPHS) to produce L-threonine (L-Thr) and inorganic phosphate. Here, we report a simplified purification protocol for the OPHS substrate and a continuous, coupled-coupled, spectrophotometric IS assay. The sequential actions of threonine deaminase (TD) and hydroxyisocaproate dehydrogenase (HO-HxoDH) convert the L-Thr product of IS to alpha-ketobutyrate (alpha-KB) and then to 2-hydroxybutyrate, respectively, and are monitored as the decrease in absorbance at 340 nm resulting from the concomitant oxidation of beta-nicotinamide adenine dinucleotide (NADH) to NAD(+) by HO-HxoDH. The effect of pH on the activities of Escherichia coli TD and Lactobacillus delbrueckii HO-HxoDH was determined to establish this continuous assay as suitable for steady-state characterization and to facilitate the optimization of coupling enzyme concentrations under different assay conditions to enable studies of IS across phyla. To validate this assay, IS from E. coli was characterized. The kinetic parameters (k(cat)=4 s(-1) and K-m = 0.34 mM) and the pH optimum of 8.7, determined using the continuous assay, are consistent with values reported for this enzyme based on the discontinuous malachite green assay. The k(cat)/K-m(OPHs) versus pH profile of E. coli IS is bell-shaped, and the apparent pK(a) values for the acidic and basic limbs are 7.1 and 10.4, respectively.

• 出版日期2012-4-1