Amyloid-beta Contributes to Blood-Brain Barrier Leakage in Transgenic Human Amyloid Precursor Protein Mice and in Humans With Cerebral Amyloid Angiopathy

作者：Hartz Anika M S; Bauer Bjoern; Soldner Emma L B; Wolf Andrea; Boy Sandra; Backhaus Roland; Mihaljevic Ivan; Bogdahn Ulrich; Kluenemann Hans H; Schuierer Gerhard; Schlachetzki Felix^*

来源：Stroke, 2012, 43(2): 514-523.

DOI：10.1161/STROKEAHA.111.627562

• amyloid-beta
• blood-brain barrier
• cerebral amyloid angiopathy
• neuroimaging

Background and Purpose-Cerebral amyloid angiopathy (CAA) is a degenerative disorder characterized by amyloid-beta (A beta) deposition in the blood brain barrier (BBB). CAA contributes to injuries of the neurovasculature including lobar hemorrhages, cortical microbleeds, ischemia, and superficial hemosiderosis. We postulate that CAA pathology is partially due to A beta compromising the BBB. %26lt;br%26gt;Methods-We characterized 19 patients with acute stroke with %26quot;probable CAA%26quot; for neurovascular pathology based on MRI and clinical findings. Also, we studied the effect of A beta on the expression of tight junction proteins and matrix metalloproteases (MMPs) in isolated rat brain microvessels. %26lt;br%26gt;Results-Two of 19 patients with CAA had asymptomatic BBB leakage and posterior reversible encephalopathic syndrome indicating increased BBB permeability. In addition to white matter changes, diffusion abnormality suggesting lacunar ischemia was found in 4 of 19 patients with CAA; superficial hemosiderosis was observed in 7 of 9 patients. A beta(40) decreased expression of the tight junction proteins claudin-1 and claudin-5 and increased expression of MMP-2 and MMP-9. Analysis of brain microvessels from transgenic mice overexpressing human amyloid precursor protein revealed the same expression pattern for tight junction and MMP proteins. Consistent with reduced tight junction and increased MMP expression and activity, permeability was increased in brain microvessels from human amyloid precursor protein mice compared with microvessels from wild-type controls. %26lt;br%26gt;Conclusions-Our findings indicate that A beta contributes to changes in brain microvessel tight junction and MMP expression, which compromises BBB integrity. We conclude that A beta causes BBB leakage and that assessing BBB permeability could potentially help characterize CAA progression and be a surrogate marker for treatment response. (Stroke. 2012;43:514-523.)

• 出版日期2012-2