Maximizing Robustness and Throughput in Liquid Chromatography by Using Pressure-Controlled Operation

作者:Verstraeten Matthias; Broeckhoven Ken; Witt Klaus; Choikhet Konstantin; Dittmann Monika; Desmet Gert*
来源:LC-GC North America, 2012, 30(12): 1046-1051.

摘要

A considerable gain in analysis time (10-30%) can be obtained by switching from a constant-flow-rate mode of operation to a constant-pressure gradient-elution mode. This switch will not reduce separation selectivity, because selectivity is volume-based and thus is independent of the flow rate. The peak areas and plate heights are similar in the two modes.

  • 出版日期2012-12