A new primer for 16S rDNA analysis of microbial communities associated with Porphyra yezoensis

作者：Namba Aki^*; Shigenobu Yuya; Kobayashi Masahiro; Kobayashi Takanori; Oohara Ichiro

来源：Fisheries Science, 2010, 76(5): 873-878.

DOI：10.1007/s12562-010-0273-z

• Porphyra yezoensis
• 16S rDNA
• Polymerase chain reaction
• Universal primer
• Microbial community

To construct high-quality 16S rDNA clone libraries for microbial communities associated with Porphyra yezoensis and to minimize the detection of rDNA from leafy gametophytes of P. yezoensis, we designed a new 16S rDNA universal primer (75F). Of the clones prepared using 75F, which was designed to distinguish between bacteria and P. yezoensis, 95% were classified into four groups, namely, beta-proteobacteria, gamma-proteobacteria, Lentisphaerae, and Flavobacteria. PCR-based analysis of the 16S rDNA primer constructed in this study can be used to implement 16S rDNA-based methodologies for the investigation of microbial community composition and diversity related to the Porphyra group.

• 出版日期2010-9