Anti-Mycobacterium activity of microbial peptides in a silkworm infection model with Mycobacterium smegmatis

作者：Yagi Akiho; Uchida Ryuji; Hamamoto Hiroshi; Sekimizu Kazuhisa; Kimura Ken ichi; Tomoda Hiroshi^*

来源：Journal of Antibiotics, 2017, 70(5): 685-690.

DOI：10.1038/ja.2017.23

An in vivo-mimic silkworm infection model with Mycobacterium smegmatis was established. When silkworms were raised at 37 degrees C following an injection of M. smegmatis cells (1.25 x 10(7) CFU larva(-1) g(-1)) into the silkworm hemolymph, they died within 48 h. Under these conditions, four microbial peptides with anti-M. smegmatis activity, lariatin A, calpinactam, lysocin E and propeptin, exerted therapeutic effects in a dose-dependent manner, and these are also clinically used agents that are active against Mycobacterium tuberculosis. These results indicate that the silkworm infection model with M. smegmatis is practically useful for the screening of therapeutically effective anti-M. tuberculosis antibiotics.

• 出版日期2017-5
• 单位河北医科大学