A transmembrane domain determines the localization of rice stripe virus pc4 to plasmodesmata and is essential for its function as a movement protein

作者：Rong, Lingling; Lu, Yuwen; Lin, Lin; Zheng, Hongying; Yan, Fei^*; Chen, Jianping

来源：Virus Research, 2014, 183: 112-116.

DOI：10.1016/j.virusres.2014.02.006

• Rice stripe virus
• pc4
• Movement
• Plasmodesmata
• Transmembrane

The pc4 protein encoded by rice stripe virus (RSV) is a viral movement protein (MP). A transmembrane (TM) domain spanning AAs 106-123 of pc4 was identified and shown to be essential for localization of pc4 to plasmodesmata (PD) (but not to chloroplasts) and for its ability to recover the movement of movement-deficient PVX. Analysis of alanine-scanning mutants showed that M116A and G120A had a similar localization to wild type pc4, being localized at PD and chloroplasts, but all other mutants were only localized at chloroplasts and not at PD. Mutants that could not be localized at PD had little (G123A) or no ability to recover PVX-GFP Delta p25 movement, indicating that PD localization is crucial for the function of pc4 as a movement protein. Strangely, mutants M116A and G120A localized at PD and retained the ability to bind single-stranded RNA but did not support PVX-GFR Delta p25 movement, indicating that properties other than PD localization and nucleotide binding ability may be needed for the function of pc4 as a movement protein.

• 出版日期2014-4-21
• 单位浙江师范大学; 浙江省农业科学院