Multi-microarray identifies lower AQP9 expression in adjuvant chemotherapy nonresponders with stage III colorectal cancer

作者：Dou, Ruoxu; Deng, Yanhong^*; Huang, Lanlan; Fu, Shijun; Tan, Shuyun; Wang, Lei; Lian, Lei; Fang, Lekun; Fan, Xinjuan; Jin, Gang; Liu, Huanliang; Wang, Jianping

来源：Cancer Letters, 2013, 336(1): 106-113.

DOI：10.1016/j.canlet.2013.04.017

• Microarray
• AQP9
• Chemosensitivity

Approximately 25% of stage III colorectal cancer patients do not benefit from standard adjuvant chemotherapies. To identify biomarkers for nonresponders, fresh-frozen tissues of 19 nonresponders and 16 responders were analyzed with gene expression and microRNA arrays. Fifty-nine genes and 17 miRNAs were differentially expressed by at least two folds. AQP9, SATB2, and WIF1 were simultaneously lower expressed in the nonresponders and modulated by the differentially expressed miRNAs. RT-PCR validated the differential expression of AQP9 (p = 0.035). In conclusion, lower AQP9 gene expression is related with non-response to adjuvant chemotherapy, showing potential as predictive marker and therapeutic target.

• 出版日期2013-8-9
• 单位上海华冠生物芯片有限公司; 中山大学