摘要
In this study, whey protein was hydrolyzed by trypsin. A calcium binding peptide was isolated and purified by ultra-filtration, gel filtration chromatography, and reverse phase HPLC from the hydrolysate. The target peptide, with a molecular weight of 555.54 Da and with the amino acid sequence Ser-Thr-Glu-Tyr-Gly, corresponds to the 48 to 52 th amino acid sequence of bovine -lactalbumin, which exhibited a significant effect on promoting the absorption of calcium in mice. The peptide could be used as a food additive to prevent calcium deficiency in humans.