A locus conferring effective late blight resistance in potato cultivar Sarpo Mira maps to chromosome XI

作者：Tomczynska Iga; Stefanczyk Emil; Chmielarz Marcin; Karasiewicz Beata; Kaminski Piotr; Jones Jonathan D G; Lees Alison K; Sliwka Jadwiga^*

来源：Theoretical and Applied Genetics, 2014, 127(3): 647-657.

DOI：10.1007/s00122-013-2248-9

Late blight of potato, caused by Phytophthora infestans, is one of the most economically important diseases worldwide, resulting in substantial yield losses when not adequately controlled by fungicides. Late blight was a contributory factor in The Great Irish Famine, and breeding for resistance to the disease began soon after. Several disease-resistant cultivars have subsequently been obtained, and amongst them Sarpo Mira is currently one of the most effective. The aim of this work was to extend the knowledge about the genetic basis of the late blight resistance in Sarpo Mira and to identify molecular markers linked to the resistance locus which would be useful for marker-assisted selection. A tetraploid mapping population from a Sarpo Mira x Maris Piper cross was phenotyped for foliar late blight resistance using detached leaflet tests. A locus with strong effect on late blight resistance was mapped at the end of chromosome XI in the vicinity of the R3 locus. Sarpo Mira's genetic map of chromosome XI contained 11 markers. Marker 45/XI exhibited the strongest linkage to the resistance locus and accounted for between 55.8 and 67.9 % of variance in the mean resistance scores noted in the detached leaflet assays. This marker was used in molecular marker-facilitated gene pyramiding. Ten breeding lines containing a late blight resistance locus from cultivar Sarpo Mira and the Rpi-phu1 gene originating from the late blight resistant accession of Solanum phureja were obtained. These lines have extended the spectrum of late blight resistance compared with Sarpo Mira and it is expected that resistance in plants containing this gene pyramid will have enhanced durability.

• 出版日期2014-3