Downregulation of miR-130a, antagonized doxorubicin-induced cardiotoxicity via increasing the PPAR gamma expression in mESCs-derived cardiac cells

作者：Pakravan Golnaz; Foroughmand Ali Mohammad^*; Peymani Maryam; Ghaedi Kamran^*; Hashemi Motahare Sadat; Hajjari Mohammadreza; Nasr Esfahani Mohammad Hossein^*

来源：Cell Death & Disease, 2018, 9(7): 758.

DOI：10.1038/s41419-018-0797-1

Doxorubicin (Dox) is a widely used powerful chemotherapeutic component for cancer treatment. However, its clinical application has been hampered due to doxorubicin-induced cardiomyopathy upon the cessation of chemotherapy. Previous studies revealed that PPAR gamma plays a crucial protective role in cardiomyocytes. Modulation of miRNA expression is an applicable approach for prohibition of toxicity induction. Therefore, the aim of present study is uprising of PPAR gamma transcript levels via manipulation of miRNAs to limit Dox-induced cardiotoxicity in mESCs-derived cardiac cells, as in vitro model cell to provide a simple direct approach for further clinical therapies. Based on bioinformatics data mining, eventually miR-130a was selected to target PPAR gamma. This miRNA is highly expressed in heart. The expression of miR-130a increases sharply upon Dox treatment while specific antagomiR-130a reverses Dox-induced reduced expression of PPAR gamma, cellular apoptosis, and inflammation. Our data strongly suggest that antagomiR-130a limits Dox-induced cellular toxicity via PPAR gamma upregulation and may have clinical relevance to limit in vivo Dox toxicity.

• 出版日期2018-7-9