A novel real-time fluorescence mutant-allele-speciric amplification method for rapid single nucleotide polymorphism analysis

作者：Zhu, DB; Xing, D^*; Li, X; Zhang, L

来源：Chinese Chemical Letters, 2006, 17(4): 499-501.

• mutant-allele-specific amplification
• single nucleotide polymorphism analysis
• SYBR Green I
• K-ras oncogene

Current methods for single nucleotide polymorphism (SNP) analysis are time-consuming and complicated. We aimed at development of one-step real-time fluorescence mutant-allele-specific amplification (MASA) method for rapid SNP analysis. The method is a marriage of two technologies: MASA primers for target DNA and a double-stranded DNA-selective fluorescent dye, SYBR Green 1. Genotypes are separated according to the different threshold cycles of the wild-type and mutant primers. K-ras oncogene was used as a target to validate the feasibility of the method. The experimental results showed that the different genotypes can be clearly discriminated by the assay. The real-time fluorescence MASA method will have an enormous potential for fast and reliable SNP analysis due to its simplicity and low cost.

• 出版日期2006-4
• 单位华南师范大学