Non-structural protein 1 of influenza viruses inhibits rapid mRNA degradation mediated by double-stranded RNA-binding protein, staufen1

作者：Cho Hana; Ahn Sang Ho; Kim Kyoung Mi; Kim Yoon Ki^*

来源：FEBS Letters, 2013, 587(14): 2118-2124.

DOI：10.1016/j.febslet.2013.05.029

• Influenza virus
• NS1
• Staufen1
• Staufen1-mediated mRNA decay (SMD)

Although non-structural protein 1 (NS1) of influenza viruses is not essential for virulence, this protein is involved in host-virus interactions, viral replication, and translation. In particular, NS1 is known to interact with the host protein, staufen1 (Stau1). This interaction is important for efficient viral replication. However, the underlying molecular mechanism by which NS1 influences the viral life cycle remains obscure. Here, we show using immunoprecipitation and artificial tethering that the N-terminus of NS1, NS1(1-73), interacts with Stau1, blocks the Stau1-Upf1 interaction, and consequently inhibits the efficiency of Stau1-mediated mRNA decay (SMD), but not nonsense-mediated-mRNA decay (NMD). The regulation of SMD efficiency by NS1 may contribute to building a more favorable cellular environment for viral replication. %26lt;br%26gt;Structured summary of protein interactions: %26lt;br%26gt;STAU1-55 physically interacts with UPF1 by anti tag-coimmunoprecipitation (View interaction) %26lt;br

• 出版日期2013-7-11