Application of NaYF4 :Yb, Er Nanoparticles as Peroxidase Mimetics in Uric Acid Detection

作者：Tang Yu Rong; Zhang Yu; Liu Rui; Su Ying Ying; Lu Yi^*

来源：Chinese Journal of Analytical Chemistry, 2013, 41(3): 330-336.

DOI：10.3724/SP.J.1096.2013.20596

• NaYF4 :Yb, Er nanoparticles
• Peroxidase mimetics
• Uric acid

The poly(acrylic acid) functionalized NaYF4 : Yb, Er nanoparticles were found to possess intrinsic peroxidase-like activity, which could catalyze oxidization of 3,3',5,5'-tetramethylbiphenyl (TMB) by H2O2 to produce a colour variation. Using TMB as substrate, we systematically studied the effect of a series of conditions, such as temperature and pH, on the catalytic activity of NaYF4 : Yb, Er nanoparticles. When combined with uricase, the proposed colorimetric method could be successfully used for the determination of uric acid. Under the optimum conditions, the determination of uric acid was in the range from 1.0x10(-5) to 2.0x10(-4) mol/L with the detection limit down to 5.3x10(-6) mol/L. Moreover, the developed method was applied to detect uric acid in serum samples and the results were in good agreement with those reported by the hospital.

• 出版日期2013-3
• 单位四川大学