Arrhythmogenic Right Ventricular Dysplasia/Cardiomyopathy Pathogenic Desmosome Mutations in Index-Patients Predict Outcome of Family Screening: Dutch Arrhythmogenic Right Ventricular Dysplasia/Cardiomyopathy Genotype-Phenotype Follow-Up Study

作者：Cox Moniek G P J^*; van der Zwaag Paul A; van der Werf Christian; van der Smagt Jasper J; Noorman Maartje; Bhuiyan Zahir A; Wiesfeld Ans C P; Volders Paul G A; van Langen Irene M; Atsma Douwe E; Dooijes Dennis; van den Wijngaard Arthur; Houweling Arjan C; Jongbloed Jan D H; Jordaens Luc; Cramer Maarten J; Doevendans Pieter A; de Bakker Jacques M T; Wilde Arthur A M; van Tintelen J Peter; Hauer Richard N W

来源：Circulation, 2011, 123(23): 2690-U87.

DOI：10.1161/CIRCULATIONAHA.110.988287

• arrhythmogenic right ventricular dysplasia
• cardiomyopathy
• desmosome
• follow-up studies
• genetics

Background-Arrhythmogenic right ventricular dysplasia/cardiomyopathy (ARVD/C) is an autosomal dominant inherited disease with incomplete penetrance and variable expression. Causative mutations in genes encoding 5 desmosomal proteins are found in approximate to 50% of ARVD/C index patients. Previous genotype-phenotype relation studies involved mainly overt ARVD/C index patients, so follow-up data on relatives are scarce.
Methods and Results-One hundred forty-nine ARVD/C index patients (111 male patients; age, 49 +/- 13 years) according to 2010 Task Force criteria and 302 relatives from 93 families (282 asymptomatic; 135 male patients; age, 44 +/- 13 years) were clinically and genetically characterized. DNA analysis comprised sequencing of plakophilin-2 (PKP2), desmocollin-2, desmoglein-2, desmoplakin, and plakoglobin and multiplex ligationdependent probe amplification to identify large deletions in PKP2. Pathogenic mutations were found in 87 index patients (58%), mainly truncating PKP2 mutations, including 3 cases with multiple mutations. Multiplex ligation-dependent probe amplification revealed 3 PKP2 exon deletions. ARVD/C was diagnosed in 31% of initially asymptomatic mutation-carrying relatives and 5% of initially asymptomatic relatives of index patients without mutation. Prolonged terminal activation duration was observed more than negative T waves in V1 to V3, especially in mutation-carrying relatives <20 years of age. In 45% of screened families, >= 1 affected relatives were identified (90% with mutations).
Conclusions-Pathogenic desmosomal gene mutations, mainly truncating PKP2 mutations, underlie ARVD/C in the majority (58%) of Dutch index patients and even 90% of familial cases. Additional multiplex ligation-dependent probe amplification analysis contributed to discovering pathogenic mutations underlying ARVD/C. Discovering pathogenic mutations in index patients enables those relatives who have a 6-fold increased risk of ARVD/C diagnosis to be identified. Prolonged terminal activation duration seems to be a first sign of ARVD/C in young asymptomatic relatives. (Circulation. 2011;123:2690-2700.)

• 出版日期2011-6-14