A triple-primer PCR approach for the sex identification of endangered Phasianidae birds

作者：Li, Wenqing; Xue, Fei; Li, Li; Li, Xiao; Yue, Bisong; Li, Jing^*

来源：European Journal of Wildlife Research, 2012, 58(1): 289-294.

DOI：10.1007/s10344-011-0576-0

• Phasianidae birds
• Triple-primer PCR
• Sex identification
• Pheasant sex-linked marker

To manage and conserve endangered Phasianidae species and to study their ecology, behavior, and population structure, sex identification is necessary. A search for the pheasant sex-linked marker (PSL) was carried out by comparing the sequences of chicken sex chromosomes. A triple-primer PCR approach was established in the present study based on a novel PSL for the sex identification of Phasianidae birds. The triple-primer system amplified a 202-bp PSL-W-specific fragment in female Phasianidae birds, whereas there is a 107-bp PSL-Z-specific fragment in the both sexes, thus making the female distinct. We also found a 102-bp AT-rich repeat sequence inserted into the PSL-W-specific region in Tetraophasis szechenyii generating a similar to 300-bp PSL-W fragment in females. The sex of 11 Phasianidae species was distinguished clearly by the triple-primer PCR approach indicating that this method is both quick and reliable and can be easily applied to a wider range of Phasianidae species. Due to the relatively small size of the amplification products, the triple-primer PCR approach can also be applied to noninvasive samples such as feathers.

• 出版日期2012-2
• 单位四川大学