Differential Diagnosis of Reactive Mesothelial Cells and Malignant Mesothelioma Cells Using the Cell Proliferation Markers Minichromosome Maintenance Protein 7, Geminin, Topoisomerase II Alpha and Ki-67

作者：Kimura Fumikazu^*; Okayasu Isao; Kakinuma Hirokuni; Satoh Yukitoshi; Kuwao Sadahito; Saegusa Makoto; Watanabe Jun

来源：Acta Cytologica, 2013, 57(4): 384-390.

DOI：10.1159/000350262

• Body cavity fluid cytology
• Immunocytochemistry
• Minichromosome maintenance protein 7
• Geminin
• Topoisomerase II alpha

Objective:The aim of this study was to evaluate whether the immunocytochemical expression of cell proliferation markers, such as minichromosome maintenance protein 7 (MCM 7), geminin, topoisomerase II alpha (topo 11 alpha) and Ki-67, which are different types of cell proliferation markers, could be useful for their differential diagnosis in reactive mesothelial cells and malignant mesothelioma cells obtained from body cavity fluids. Study Design: Samples diagnosed and later histologically confirmed as reactive mesothelial cells (39 cases) or malignant mesothelioma (32 cases) in body cavity fluids were examined. Immunocytochemical staining of MCM 7, geminin, topo Il alpha and Ki-67 was performed with the immunoperoxidase polymer method. Results: Labeling indices (Lls) of MCM 7 (cutoff value 20.0%; sensitivity 100%; specificity 100%), geminin (cutoff value 4.5%; sensitivity 88.0%; specificity 70.0%), topo Ila (cutoff value 11.0%; sensitivity 88.0%; specificity 92.0%) and Ki-67 (cutoff value 15.3%; sensitivity 78.0%; specificity 79.0%) of malignant nnesothelioma cells were significantly higher than those of reactive mesothelial cells. Conclusion: Lls of MCM 7, geminin and topo Ila can be reliable tools for the differential diagnosis of reactive mesothelial cells and malignant mesothelioma cells.

• 出版日期2013