Application of response surface methodology to optimize nitrate removal at low temperature by aerobic denitrificator Pseudomonas strain An-1

作者：Ren, He-Jun; Su, Yu; Wang, Cong; Hou, Zhi-Min; Zhou, Ye; Zhou, Rui^*

来源：Water and Environment Journal, 2018, 32(2): 235-241.

DOI：10.1111/wej.12319

• biological denitrification
• groundwater
• low temperature
• nitrate removal
• response surface methodology

Response surface methodology (RSM) was applied to optimize the conditions of nitrate removal by Pseudomonas strain AN-1 at low temperature, which was isolated from aniline-contaminated groundwater. A three-factor, three-level Box-Behnken experimental design and analysis of variance were combined with the obtained second-order polynomial equation and applied for RSM optimization. By solving the second-order polynomial equation and analysing the 3D response surface, a NO3--N concentration of 65.29 mg/L, an initial pH of 6.65, and an initial DO of 7.53 were identified as the optimized conditions. These conditions achieved a removal percentage of 53.7485% after 48 h at 10 degrees C, which was in good agreement with the predicted 53.8085%. This study showed the excellent ability of the Pseudomonas strain AN-1 to degrade nitrate at low temperatures, which provided a solid foundation for the further application of this microorganism in the treatment of nitrate-contaminated groundwater.

• 出版日期2018-5
• 单位吉林大学; 吉林农业大学