Analysis of the Heterocyclic Aromatic Amines in Cigarette Smoke by Liquid Chromatography-Tandem Mass Spectrometry

作者：Zhao Ge^*; Wang Sheng; Fu Yufeng; Yu Jingjing; Wang Bing; Xie Fuwei; Xie Jianping

来源：Chromatographia, 2014, 77(11-12): 813-820.

DOI：10.1007/s10337-014-2687-8

• HPLC-MS/MS
• Heterocyclic aromatic amines
• Cigarette smoke
• Matrix effects

In this paper, a sensitive accurate method for the determination of heterocyclic aromatic amines (HAAs) in cigarette smoke has been developed and validated using solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry. A calibration curve was obtained with representative cigarette smoke using the standard addition method to compensate for matrix effects because the smoke of different cigarettes shows similar matrix effects. With this method, the accuracy of the method can be improved using a common analog of HAAs, which will greatly reduce the expense of using an isotope-labeled internal standard. Validation results showed that the method has high sensitivity (quantification limits of 0.08-0.56 ng cig(-1)), good reproducibility (RSD 6.37-9.31 %) and satisfactory recoveries (81.0-111 %). With this method, the emissions of HAAs in 30 commercial cigarette samples were analyzed and compared.

• 出版日期2014-6
• 单位中国烟草总公司郑州烟草研究院