SpOT the Correct Tissue Every Time in Multi-tissue Blocks

作者：Coffey Anna; Johnson Michael D; Berry Deborah L^*

来源：Jove-Journal of Visualized Experiments, 2015, (99): e52868.

DOI：10.3791/52868

• Structural Biology
• Issue 99
• Tissue Microarrays
• Multi-Tissue Paraffin Blocks
• Tissue Orientation
• Histology
• Immunohistochemistry

Multi-tissue paraffin blocks provide high throughput analysis with increased efficiency, experimental uniformity, and reduced time and cost. Tissue microarrays make up the majority of multi-tissue paraffin blocks, but increasingly, researchers are using non-arrayed blocks containing larger tissues from multiple individuals which can provide many of the advantages of tissue microarrays without substantial investment in planning and equipment. A critical component of any multi-tissue analysis is the orientation method used to identify each individual tissue. Although methods exist to maintain proper orientation and identification of tissues in multi-tissue blocks, most are not well-suited to non-arrayed blocks, may consume valuable space within an array and/or are difficult to produce in the standard histology laboratory. The Specimen Orientation Tag (SpOT) is a simple, low cost orientation tool that is clearly visible in paraffin blocks and all tissue sections for reliable specimen identification in arrayed and non-arrayed layouts. The SpOT provides advantages over existing orientation methods for non-arrayed blocks as it does not require any direct modification to the tissue and allows for flexibility in the arrangement of tissue pieces.

• 出版日期2015-5