A simple and efficient micrografting method for stably transformed Nicotiana attenuata plants to examine shoot-root signaling

作者：Fragoso Variluska; Goddard Hannah; Baldwin Ian T; Kim Sang Gyu^*

来源：Plant Methods, 2011, 7(1): 34.

DOI：10.1186/1746-4811-7-34

• Grafting
• Nicotiana attenuata
• root and shoot signaling
• systemic signals

To adjust their development to the environment, plants rely on specific signals that travel from shoot to root and vice versa. Here we describe an efficient micrografting protocol for Nicotiana attenuata, a useful tool for identifying these signals and understanding their functions. Additionally we analyzed transcript accumulation profiles of scions and rootstocks of grafts performed with wild-type and stably transformed N. attenuata. Our results are consistent with the source-to-sink movement of an sRNA silencing signal.

• 出版日期2011-10-20