In vitro regeneration of plantlets from internodal callus culture of Vitex leucoxylon L. - A rare medicinal plant

作者：Chordia M Amarchand^*; Kumari R Sangeetha; Kannan R Ramesh

来源：Journal of Medicinal Plants Research, 2010, 4(22): 2399-2403.

• Vitex leucoxylon
• internodal culture
• organogenesis
• rooting

Attempt was made to standardize the regeneration protocol. Medium was optimized for callusing, organogenesis and rooting of Vitex leucoxylon. Compact and greenish callus from internodal segments was achieved when stem segments were cultured on Murashige and Skoog (MS) medium fortified with 5.37 mu M NAA + 2.22 mu M BAP. In vitro regeneration of shoot buds was achieved from internodal callus cultured on MS medium supplemented with 13.30 mu M BAP along with 5.37 mu M NAA and/or 8.90 mu M KN with 2.69 mu M NAA. Regeneration and multiplication rate was high in MS medium containing 8.64 mu M GA(3) and 7.20 mu M BAP. Elongated shoots were isolated and cultured on strength MS medium supplemented with different concentrations of indole 3-butyric acid (IBA) for root induction. Higher degree of rooting was obtained on MS with 4.90 mu M IBA. Rooted plantlets were hardened and successfully established using garden soil. This is the first report dealing with in vitro regeneration system for V. leucoxylon L.

• 出版日期2010-11-18