A New Bilayer Chitosan Scaffolding as a Dural Substitute: Experimental Evaluation

作者：Humberto Sandoval Sanchez Jose; Ramos Zuniga Rodrigo^*; Luquin de Anda Sonia; Lopez Dellamary Fernando; Gonzalez Castaneda Rocio; De la Cruz Ramirez Jaimes Juan; Jorge Espinoza Guadalupe

来源：World Neurosurgery, 2012, 77(3-4): 577-582.

DOI：10.1016/J.WNEU.2011.07.007

• Biomaterials
• Chitosan
• Dura mater
• Duraplasty
• Graft
• Tissue engineering

OBJECTIVE: To evaluate whether bilayer chitosan scaffolding (BChS) can provide a watertight dural closure and permit regeneration by fibroblasts in an experimental in vivo model.
METHODS: In the in vitro phase, BChS was elaborated and the following characteristics were evaluated: pore size, thickness, water absorption capacity, tensile strength, strain, and toughness. In the second in vivo phase, 27 durectomized New Zealand rabbits were randomly assigned into three duraplasty groups with autologous dura, collagen matrix (CM), or BChS. In all groups, fluid leakage pressure was measured at 10, 21, or 180 days. Histology response to regeneration was evaluated through hematoxylin and eosin stain.
RESULTS: BChS was standardized to obtain bilayer scaffoldings with a nonporous layer and a porous layer. The pore size was 10 mu m, total thickness was 400 mu m, strain was 57.8%, and tensile strength was 5.5 gr/mm(2). The physical characteristics of BChS allowed dural closure without cerebrospinal fluid (CSF) leak. There were no differences in fluid leakage pressures between the BChS, dura, and CM groups. Histologic analysis showed fibroblast migration with adequate dural regeneration.
CONCLUSIONS: BChS is an ideal alternative for a watertight dural closure because it can be sutured, and it induces organized regeneration with fibroblasts without evidence of fibrosis.

• 出版日期2012-4