A Simplified Seed Transformation Method for Obtaining Transgenic Brassica napus Plants

作者：Song Li; Zhao De gang^*; Wu Yong jun; Tian Xiao e

来源：Agricultural Sciences in China, 2009, 8(6): 658-663.

DOI：10.1016/S1671-2927(08)60261-8

• Brassica napus
• seed transformation
• sonication

We report here a seed transformation of sonication-assisted, no-tissue culture to rapidly produce transgenic Brassica napus plants. This method comprises the steps of treating seeds by ultrasonic wave, inoculating Agrobacterium tumefaciens with a recombinant Ch1FN-alpha gene and germinating directly of treatment seed on wet filter papers. The obtained transformants were verified by GUS histochemical assay and nested PCR amplification. It suggests that seed transformation has a potential use in genetic transformation of rape.

• 出版日期2009-6-20
• 单位贵州大学