A novel cyanide-inducible gene cluster helps protect Pseudomonas aeruginosa from cyanide

作者：Frangipani Emanuela^*; Perez Martinez Isabel; Williams Huw D; Cherbuin Gaetan; Haas Dieter

来源：Environmental Microbiology Reports, 2014, 6(1): 28-34.

DOI：10.1111/1758-2229.12105

Pseudomonas aeruginosa produces the toxic secondary metabolite hydrogen cyanide (HCN) at high cell population densities and low aeration. Here, we investigated the impact of HCN as a signal in cell-cell communication by comparing the transcriptome of the wild-type strain PAO1 to that of an HCN-negative mutant under cyanogenic conditions. HCN repressed four genes and induced 12 genes. While the individual functions of these genes are unknown, with one exception (i.e. a ferredoxin-dependent reductase), a highly inducible six-gene cluster (PA4129-PA4134) was found to be crucial for protection of P.aeruginosa from external HCN intoxication. A double mutant deleted for PA4129-PA4134 and cioAB (encoding cyanide-insensitive oxidase) did not grow with 100M KCN, whereas the corresponding single mutants were essentially unaffected, suggesting a synergistic action of the PA4129-PA4134 gene products and cyanide-insensitive oxidase.

• 出版日期2014-2