A Janus-paper PDMS platform for air-liquid interface cell culture applications

作者：Rahimi Rahim^*; Ochoa Manuel; Donaldson Amy; Parupudi Tejasvi; Dokmeci Mehmet R; Khademhosseini Ali; Ghaemmaghami Amir; Ziaie Babak

来源：Journal of Micromechanics and Microengineering, 2015, 25(5): 055015.

DOI：10.1088/0960-1317/25/5/055015

• paper
• rapid prototyping
• PDMS bonding
• cell culture
• air-liquid interface

A commercially available Janus paper with one hydrophobic (polyethylene-coated) face and a hygroscopic/hydrophilic one is irreversibly bonded to a polydimethylsiloxane (PDMS) substrate incorporating microfluidic channels via corona discharge surface treatment. The bond strength between the polymer-coated side and PDMS is characterized as a function of corona treatment time and annealing temperature/time. A maximum strength of 392 kPa is obtained with a 2 min corona treatment followed by 60 min of annealing at 120 degrees C. The water contact angle of the corona-treated polymer side decreases with increased discharge duration from 98 degrees to 22 degrees. The hygroscopic/hydrophilic side is seeded with human lung fibroblast cells encapsulated in a methacrylated gelatin (GelMA) hydrogel to show the potential of this technology for nutrient and chemical delivery in an air-liquid interface cell culture.

• 出版日期2015-5