Acacia honey accelerates in vitro corneal ulcer wound healing model

作者：Abd Ghafar, Norzana^*; Ker-Woon, Choy; Hui, Chua Kien; Yusof, Yasmin Anum Mohd; Ngah, Wan Zurinah Wan

来源：BMC Complementary and Alternative Medicine, 2016, 16(1): 259.

DOI：10.1186/s12906-016-1248-0

• Acacia honey
• Corneal fibroblasts
• In vitro corneal ulcer
• Wound healing

Background: The study aimed to evaluate the effects of Acacia honey (AH) on the migration, differentiation and healing properties of the cultured rabbit corneal fibroblasts.
Methods: Stromal derived corneal fibroblasts from New Zealand White rabbit (n = 6) were isolated and cultured until passage 1. In vitro corneal ulcer was created using a 4 mm corneal trephine onto confluent cultures and treated with basal medium (FD), medium containing serum (FDS), with and without 0.025 % AH. Wound areas were recorded at day 0, 3 and 6 post wound creation. Genes and proteins associated with wound healing and differentiation such as aldehyde dehydrogenase (ALDH), vimentin, alpha-smooth muscle actin (alpha-SMA), collagen type I, lumican and matrix metalloproteinase 12 (MMP12) were evaluated using qRT-PCR and immunocytochemistry respectively.
Results: Cells cultured with AH-enriched FDS media achieved complete wound closure at day 6 post wound creation. The cells cultured in AH-enriched FDS media increased the expression of vimentin, collagen type I and lumican genes and decreased the ALDH, alpha-SMA and MMP12 gene expressions. Protein expression of ALDH, vimentin and a-SMA were in accordance with the gene expression analyses.
Conclusion: These results demonstrated AH accelerate corneal fibroblasts migration and differentiation of the in vitro corneal ulcer model while increasing the genes and proteins associated with stromal wound healing.

• 出版日期2016-7-29