Application of response surface methodology for glucan production from Leuconostoc dextranicum and its structural characterization

作者：Majumder Avishek; Singh Angad; Goyal Arun^*

来源：Carbohydrate Polymers, 2009, 75(1): 150-156.

DOI：10.1016/j.carbpol.2008.07.014

• Leuconostoc dextranicum
• Glucan
• Plackett-Burman
• Box-Behnken design
• Exopolysaccharide
• NMR

Sequential optimization strategy based on statistical experimental designs was employed to enhance glucan production by Leuconostoc dextranicum NRRL B-1146 in flask culture. A two-level Plackett-Burman design was employed first where I I variables were studied for their influence on glucan production. Sucrose, peptone and yeast extract were the most significant variables improving glucan production. A three-level Box-Behnken factorial design was employed for maximizing the glucan production. A mathematical model was developed to show the effects of each medium component and their combinatorial interactions on glucan production. The optimal medium composition for maximum glucan production was sucrose 5.95%, peptone 0.52% and yeast extract 2.9%. This composition predicted 1063 mg/l glucan, the experimentally found glucan was 1015 +/- 4.5 mg/l that showed a good agreement with the predicted value. The purified glucan was homogenous and its structural characteristics investigated by FT-IR. H-1 NMR and C-13 NMR spectroscopic techniques showed that it contained alpha-(1 -> 6) and alpha-(1 -> 4) linkages.

• 出版日期2009-1-5