A primary investigation for the function of UbcH10, a cancer-promoting gene, in TPC-1 thyroid cancer cells

作者：Tian, Wuguo; Hao, Shuai; Li, Zhirong; Chen, Yi; Wang, Lingli; Jiang, Yan; Luo, Donglin^*

来源：International Journal of Clinical and Experimental Medicine, 2018, 11(2): 593-602.

• UbcH10
• thyroid cancer
• PI3K/Akt pathway
• TPC-1 cells

Thyroid cancer is the most common and highly aggressive endocrine malignancy. Recently, UbcH10 was correlated with this disease, however, the exact role of UbcH10 in the development of thyroid cancer remains unknown. In this study, the effect of UbcH10 knockdown by siUbcH10 on apoptosis, proliferation, and other cellular functions of thyroid cancer cell line TPC-1 was evaluated. The results showed that UbcH10 knockdown induced apoptosis and inhibition of proliferation. The outcomes of the cells cycle detection showed that the percent of G2/M phase of TPC-1 cells transfected with siUbcH10 enhanced from 5.0% to 20.68%. In addition, siRNA induced UbcH10 knockdown also resulted in a reduction in migration and invasion of TPC-1 cells. Moreover, transfection with siUbcH10 suppressed the activation of PI3K/Akt pathway, evidenced by the decreased levels of phosphorylated PI3K and Akt. In conclusion, UbcH10 may be a tumor promoter and a potential therapeutic target for the clinical management of thyroid cancer.

• 出版日期2018