A novel virus of the late blight pathogen, Phytophthora infestans, with two RNA segments and a supergroup 1 RNA-dependent RNA polymerase

作者：Cai Guohong; Myers Kevin; Hillman Bradley I; Fry William E^*

来源：Virology, 2009, 392(1): 52-61.

DOI：10.1016/j.virol.2009.06.040

• Oomycete
• Stramenopile
• RNA virus
• Fungal virus
• Astrovirus
• Potyvirus
• Potato late blight

Double-stranded RNA representing four distinct electrophoretic patterns was found in a screen of Phytophthora infestans isolates. Two dsRNAs that always appeared together were sequenced. RNA 1, which was 3160 nt Plus a poly (A) tail, contained a single deduced ORE with the potential to encode a polyprotein of 977 aa with motifs characteristic of supergroup I viral RdRps. The 2776 nt, polyadenylated RNA2 contained an ORF with a potential to encode a polyprotein of 847 aa including a possible trypsin-like serine protease, and a second putative ORF of unknown function. An alternative form of RNA2, in which a 19-nt stretch was replaced by a 9-nt sequence, was detected in 4 of 17 clones sequenced. Based on genome Structure and phylogenetic analysis, this virus did not fit into any known Virus family and we tentatively named it Phytophthora infestans RNA Virus 1 (PiRV-1).

• 出版日期2009-9-15