An arylboronate-based fluorescent probe for selective and sensitive detection of peroxynitrite and its applications for fluorescence imaging in living cells

作者：Zhang, Jian; Li, Yaping; Zhao, Jingjing; Guo, Wei^*

来源：Sensors and Actuators B: Chemical , 2016, 237: 67-74.

DOI：10.1016/j.snb.2016.06.054

• Fluorescent probes
• Peroxynitrite
• Arylboronate
• Iminocourmarin
• Bioimaging
• Oxygen-glucose deprivation

Peroxynitrite (ONOO-) is an extremely reactive oxygen species in biological systems, and can react with a wide variety of molecular targets including proteins, lipids, and nucleic acids, eventually resulting in mitochondria dysfunction and cell death. In this work, we designed and synthesized a new arylboronate-based fluorescent probe, which could detect ONOO- with a big fluorescence off-on ratio (155-fold), fast response rate (50 s), high sensitivity (detection limit:0.83 nM), and excellent selectivity over a series of biologically relevant reactive oxygen species as well as anions, cations, and biothiols. The sensing mechanism has proved to be the ONOO--triggered covalent assembly of a bright iminocourmarin fluorophore. With the probe, the stimulation-induced ONOO- in RAW264.7 murine macrophages as well as the endogenous ONOO- in endothelial cells after oxygen-glucose deprivation (OGD) has been successfully visualized.

• 出版日期2016-12
• 单位山西大学