A genome-wide SNP-based genetic map and QTL mapping for agronomic traits in Chinese cabbage

作者：Huang, Li; Yang, Yafei; Zhang, Fang; Cao, Jiashu^*

来源：Scientific Reports, 2017, 7(1): 46305.

DOI：10.1038/srep46305

• MARKER-ASSISTED SELECTION
• BRASSICA-RAPA
• LINKAGE MAP
• LOCI
• SEQUENCE
• CONSTRUCTION
• GENERATION
• RESISTANCE
• CAMPESTRIS
• DISCOVERY

The aim of this work was to construct a high-resolution genetic map for the dissection of complex morphological and agronomic traits in Chinese cabbage (Brassica rapa L. syn. B. campestris). Chinese cabbage, an economically important vegetable, is a good model plant for studies on the evolution of morphologic variation. Herein, two high-generation inbred Chinese cabbage lines, 'Huangxiaoza' and 'Bqq094-11', were crossed. Then restriction-site-associated DNA sequencing (RAD-seq) was performed on the parents and 120 F-2 individuals. A genetic map containing 711 bins representing 3985 single nucleotide polymorphism (SNP) markers was constructed. By using WinQTL with composite interval mapping (CIM) and mixed-model based composite interval mapping (MCIM) analysis via QTLNetwork, quantitative trait loci (QTL) linked to 16 genetic traits related to plant size, color and leaf characteristics were mapped to 10 linkage groups. The high density genetic map and QTL identified for morphological and agronomic traits lay the groundwork for functional gene mapping, map-based cloning and marker-assisted selection (MAS) in Chinese cabbage.

• 出版日期2017-4-18
• 单位浙江大学