Airway microbiota across age and disease spectrum in cystic fibrosis

作者：Zemanick Edith T^*; Wagner Brandie D; Robertson Charles E; Ahrens Richard C; Chmiel James F; Clancy John P; Gibson Ronald L; Harris William T; Kurland Geoffrey; Laguna Theresa A; McColley Susanna A; McCoy Karen; Retsch Bogart George; Sobush Kurtis T; Zeitlin Pamela L; Stevens Mark J; Accurso Frank J; Sagel Scott D; Harris J Kirk

来源：European Respiratory Journal, 2017, 50(5): 1700832.

DOI：10.1183/13993003.00832-2017

Our objectives were to characterise the microbiota in cystic fibrosis (CF) bronchoalveolar lavage fluid (BALF), and determine its relationship to inflammation and disease status.
BALF from paediatric and adult CF patients and paediatric disease controls undergoing clinically indicated bronchoscopy was analysed for total bacterial load and for microbiota by 16S rDNA sequencing.
We examined 191 BALF samples (146 CF and 45 disease controls) from 13 CF centres. In CF patients aged < 2 years, nontraditional taxa (e. g. Streptococcus, Prevotella and Veillonella) constituted similar to 50% of the microbiota, whereas in CF patients aged. >= 6 years, traditional CF taxa (e. g. Pseudomonas, Staphylococcus and Stenotrophomonas) predominated. Sequencing detected a dominant taxon not traditionally associated with CF (e. g. Streptococcus or Prevotella) in 20% of CF BALF and identified bacteria in 24% of culturenegative BALF. Microbial diversity and relative abundance of Streptococcus, Prevotella and Veillonella were inversely associated with airway inflammation. Microbiota communities were distinct in CF compared with disease controls, but did not differ based on pulmonary exacerbation status in CF.
The CF microbiota detected in BALF differs with age. In CF patients aged < 2 years, Streptococcus predominates, whereas classic CF pathogens predominate in most older children and adults.

• 出版日期2017-11-1