Discovery of SNPs in RXFP2 related to horn types in sheep

作者：Wang, Xiaolong; Zhou, Guangxian; Li, Qiang; Zhao, Dengfei; Chen, Yulin^*

来源：Small Ruminant Research, 2014, 116(2-3): 133-136.

DOI：10.1016/j.smallrumres.2013.10.022

• RXFP2
• Horn morphology
• Poll
• Domestic sheep

This study was conducted with an attempt to reveal genetic variation in ovine RXFP2, which is a strong candidate gene that is associated with sheep horn development and types. All 18 exons and intro-exon boundary regions as well as 5' and 3' gene flanking regions of RXFP2 were re-sequenced using DNA panels representing four typical horn types (normal-horned males, normal-horned females, scurred females, and polled females) in Tan sheep, as well as the DNA pools from Suffolk, a polled sheep breed. A total of 20 previously unreported SNPs were detected. One in the 5'UTR, four in exons, and 15 in the introns. Seven of the 20 SNPs were species-specific variants. A synonymous mutation, p.P375 (c.1125A>G), demonstrating distinct genotypes between horned and hornless individuals, which is located in a conserved region, was regarded as a candidate variant indicating the appearance or absence of horns in sheep.

• 出版日期2014-2
• 单位西北农林科技大学