Highly sensitive profiling of CD44(+)/CD24(-) breast cancer stem cells by combining global mRNA amplification and next generation sequencing: Evidence for a hyperactive PI3K pathway

作者：Hardt Olaf; Wild Stefan; Oerlecke Ilka; Hofmann Kay; Luo Shujun; Wiencek Yvonne; Kantelhardt Eva; Vess Christoph; Smith Geoffrey P; Schroth Gary P; Bosio Andreas; Dittmer Juergen^*

来源：Cancer Letters, 2012, 325(2): 165-174.

DOI：10.1016/j.canlet.2012.06.010

• Breast cancer
• Cancer stem cells
• CD44
• TGFbeta
• Next generation sequencing
• PI3K

We performed next generation sequencing- and microarray-based gene expression profiling of CD44(+)/CD24(-)/CD45(-) breast CSCs (cancer stem cells) isolated from primary ER alpha-positive breast cancer. By combining semi-automated dissociation of human tumor tissue, magnetic cell sorting and cDNA amplification less than 500 CSCs were required for transcriptome analyses. Besides overexpressing genes involved in maintenance of sternness, the CSCs showed higher levels of genes that drive the PI3K pathway, including EGFR, HB-EGF, PDGFRA/B, PDGF, MET, PIK3CA, PIK3R1 and PIK3R2. This suggests that, in CSCs of ER alpha-positive breast cancer, the PI3K pathway which is involved in endocrine resistance is hyperactive.

• 出版日期2012-12-28