Antinociceptive Effect of Crude Extract, Fractions and Three Alkaloids Obtained from Fruits of Piper tuberculatum

作者：Rodrigues Rosely Valeria; Lanznaster Debora; Longhi Balbinot Daniela Tagliari; Gadotti Vinicius de Maria; Facundo Valdir Alves; Soares Santos Adair Roberto

来源：Biological & Pharmaceutical Bulletin, 2009, 32(10): 1809-1812.

DOI：10.1248/bpb.32.1809

• Piper tuberculatum
• Piperaceae
• alkaloid
• pain
• acetic acid

This study investigated the possible antinociceptive effect of the crude extract, fractions and pure compounds (three alkaloids) obtained from fruits of Piper tuberculatum JACQ. (Piperaceae) in acetic acid-induced visceral pain in mice. Oral administration of crude extract and fractions (CH2Cl2, EtOAc, methanol and hexane) (3-300 mg kg(-1)) caused a dose-related and significant inhibition of the acetic acid-induced visceral nociceptive response. The crude extract, dichloromethane (CH2Cl2) and ethyl acetate (EtOAc) fractions were more potent than methanol and hexane fractions. The isolated alkaloids dihydro-piplartine, piplartine and 3,4,5-trimethoxydihydrocinnamic acid (0.0001-30 mg kg(-1)) exhibited significant and dose-related antinociceptive effects against acetic acid-induced visceral pain. The results show, for the first time, that crude extract, fractions and pure compounds obtained from P tuberculatum produce marked antinociception against the acetic acid-induced visceral nociceptive response, supporting the ethnomedical use of P. tuberculatum.

• 出版日期2009-10