Microchip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate salts

作者：Gunasekara Dulan B; Hulvey Matthew K; Lunte Susan M; Fracassi da Silva Jose Alberto

来源：Analytical and Bioanalytical Chemistry, 2012, 403(8): 2377-2384.

DOI：10.1007/s00216-012-5810-4

• Nitric oxide
• Microchip electrophoresis
• Reactive nitrogen species
• Amperometric detection
• NONOates

Microchip electrophoresis (ME) with electrochemical detection was used to monitor nitric oxide (NO) production from diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) and 1-(hydroxyl-NNO-azoxy)-l-proline disodium salt (PROLI/NO). NO was generated through acid hydrolysis of these NONOate salts. The products of acid hydrolysis were introduced into a 5-cm separation channel using gated injection. The separation was accomplished using reverse polarity and a background electrolyte consisting of 10 mM boric acid and 2 mM tetradecyltrimethylammonium bromide, pH 11. Electrochemical detection was performed using an isolated potentiostat in an in-channel configuration. Potentials applied to the working electrode, typically higher than +1.0 V vs. Ag/AgCl, allowed the direct detection of nitrite, NO, DEA/NO, and PROLI/NO. Baseline resolution was achieved for the separation of PROLI/NO and NO while resolution between DEA/NO and NO was poor (1.0 +/- 0.2). Nitrite was present in all samples tested.

• 出版日期2012-6