A DELAYED RECTIFIER POTASSIUM CHANNEL CLONED FROM BOVINE ADRENAL-MEDULLA - FUNCTIONAL-ANALYSIS AFTER EXPRESSION IN XENOPUS OOCYTES AND IN A NEUROBLASTOMA CELL-LINE

作者：GARCIAGUZMAN M; SALA F; CRIADO M; SALA S

来源：FEBS Letters, 1994, 354(2): 173-176.

DOI：10.1016/0014-5793(94)01117-6

• CDNA CLONING
• BOVINE ADRENAL MEDULLA
• POTASSIUM CHANNEL
• STABLE TRANSFECTION
• HETEROLOGOUS EXPRESSION

Using a cDNA library from bovine adrenal medulla, and, subsequently, a bovine genomic library, we have isolated the gene coding for a non inactivating potassium channel. This gene encodes a 597-amino acid protein which we have called BAK5 as its sequence is very similar to members of Kv1.5 potassium channel family. Neuroblastoma cells (Neuro-2a cell line) were stably transfected with BAK5 DNA. Protein expression was under the control of a heat-shock promoter. Transfected cells showed a current highly selective for potassium, insensitive to tetraethylammonium but reversibly blocked by 4-aminopyridine. Oocytes injected with BAK5 mRNA also expressed a potassium current with the same characteristics.

• 出版日期1994-11-7