Short-term anti-vascular endothelial growth factor treatment elicits vasculogenic mimicry formation of tumors to accelerate metastasis

作者：Xu, Yuan; Li, Qin; Li, Xiao Yu; Yang, Qiu Ya; Xu, Wei Wei; Liu, Gao Lin^*

来源：Journal of Experimental & Clinical Cancer Research, 2012, 31(1): 16.

DOI：10.1186/1756-9966-31-16

• Antiangiogenic therapies
• Metastasis
• Hypoxia
• Vasculogenic mimicry

Background: Antiangiogenic therapy is one of the most significant advances in anticancer treatment. The benefits of antiangiogenic therapies of late-stage cancers have been investigated but are still too limited.
Methods: We used an ovarian cancer model to test the effect of short-term bevacizumab treatment on metastasis as measured by bioluminescence. Western blotting and CD34-PAS dual staining were performed to assess hypoxia-inducible transcription factor-1 alpha (HIF-1 alpha) expression and vasculogenic mimicry(VM) formation. Cell viability was examined by a CCK8 assay.
Results: Bevacizumab demonstrated antitumor effects in models of ovarian cancer, but also accelerated metastasis together, with marked hypoxia and VM formation in mice receiving short-term therapy. Bevacizumab treatment did not affect SKOV3 cell viability and the amount of VM in three-dimensional culture.
Conclusion: These results suggest that antiangiogenic therapy may potentially influence the progression of metastatic disease, which has been linked to the hypoxic response and VM formation.

• 出版日期2012-2-23
• 单位上海交通大学