Unremitting Cell Proliferation in the Secretory Phase of Eutopic Endometriosis: Involvement of pAkt and pGSK3 beta

作者：Franco Murillo Yanira; Antonio Miranda Rodriguez Jose; Rendon Huerta Erika; Montano Luis F; Velazquez Cornejo Gerardo; Poblano Gomez Lucila; Javier Valdez Morales Francisco; Gonzalez Sanchez Ignacio; Cerbon Marco^*

来源：Reproductive Sciences, 2015, 22(4): 502-510.

DOI：10.1177/1933719114549843

• stem cell factor
• c-kit receptor
• eutopic endometrium
• endometriosis
• Akt
• GSK3

Objective: Endometriosis is linked to altered cell proliferation and stem cell markers c-kit/stem cell factor (SCF) in ectopic endometrium. Our aim was to investigate whether c-kit/SCF also plays a role in eutopic endometrium.
Design: Eutopic endometrium obtained from 35 women with endometriosis and 25 fertile eumenorrheic women was analyzed for in situ expression of SCF/c-kit, Ki67, RAC-alpha serine/threonine-protein kinase (Akt), phosphorylated RAC-alpha serine/threonin-protein kinase (pAkt), Glycogen synthase kinase 3 beta (GSK3), and phosphorylated glycogen synthase kinase 3 beta (pGSK3), throughout the menstrual cycle.
Results: Expression of Ki67 and SCF was higher in endometriosis than in control tissue (P < .05) and greater in secretory rather than proliferative (P < .01) endometrium in endometriosis. Expression of c-kit was also higher in endometriosis although similar in both phases. Expression of Akt and GSK3 was identical in all samples and cycle phases, whereas pAkt and pGSK3, opposed to control tissue, remained overexpressed in the secretory phase in endometriosis.
Conclusion: Unceasing cell proliferation in the secretory phase of eutopic endometriosis is linked to deregulation of c-kit/SCF-associated signaling pathways.

• 出版日期2015-4